Antigen-specific responses assessment for the evaluation ofBordetella pertussisT cell immunity in humans

Highlights * The assessment of T cell responses is technically complex and usually performed on fresh PBMC. * To identify simplified methods to assess pertussis specific T cell responses. * High concordance in proliferation results was obtained when CFSE and blast read-outs were compared. * Pertussis-specific assays performed on fresh PBMC were compared to frozen PBMC from the same donor. * Results from fresh and frozen PBMC did not show significant difference. * Adopting blast analysis and frozen PBMC, the possibility to test T cell responses is simplified.