Mise au point et validation d'une méthode de dosage du Méthanol dans le sang par Chromatographie en Phase Gazeuse avec injecteur « Headspace » couplée à un Détecteur à Ionisation de Flamme/[ Validation of a quantitative determination method of methanol in blood using Gas Chromatography with Flame Ionization detection after a Headspace sampling ]

Methanol is a commonly used organic solvent that, because of its toxicity, can cause metabolic acidosis, neurologic sequelae, and even death, when ingested. It is a constituent of many commercially available industrial solvents and of poorly adulterated alcoholic beverages. The management of acute human methanol intoxication needs methanol dosage. The aim of this study is to validate a method in the order to determinate the amount of blood methanol, using gas chromatography with flame ionization detection, after a Headspace sampling. The researchers used butanol as an internal standard. The validation procedure was performed according to the guidelines of the French Society of Analytical Toxicology and the French Committee of Accreditation. The intra- and inter-day precision and relative bias were less than 15 %. The limit of detection was 0.060 g/L. In the same conditions, limit of quantification was 0.09 g/L. The storage's and transport's samples were at +4 degrees Celsius for one week as a maximum.