Enteral vaccination of rats againstFasciola hepaticausing recombinant cysteine proteinase (cathepsin L1)

Cysteine proteinases released byFasciola hepaticaplay a key role in parasite feeding, migration through host tissues and in immune evasion. Hence, a recombinant cysteine proteinase (CPFhW) expressed as inclusion bodies inEscherichia coliwas used for enteral vaccination of rats against fasciolosis. W...

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Veröffentlicht in:Vaccine 2007-05, Vol.25 (18), p.3619
Hauptverfasser: Kesik, Malgorzata, Jedlina-Panasiuk, Luiza, Kozak-Cieszczyk, Monika, Plucienniczak, Andrzej, Wedrychowicz, Halina
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Sprache:eng
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Zusammenfassung:Cysteine proteinases released byFasciola hepaticaplay a key role in parasite feeding, migration through host tissues and in immune evasion. Hence, a recombinant cysteine proteinase (CPFhW) expressed as inclusion bodies inEscherichia coliwas used for enteral vaccination of rats against fasciolosis. We managed to activate this proteinase and found it to have cathepsin L1-like substrate preference. Enteral vaccination of rats induced a 78-80% protection against challenge with fluke metacercariae (mc). The immunised rats showed clear immunological response. The challenge with mc caused a remarkable infiltration of eosinophils into the peritoneal cavity of both the vaccinated rats and challenge control rats. However, CD8+ and CD4+ lymphocytes appeared in significantly higher numbers in the peritoneal fluid of vaccinated rats than in controls.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.01.057