The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea

Purpose Paclitaxel is a cytoskeletal agent that interacts with mitotic activity and inhibits microtuble disassembly. Consequently its antiproliferative effect has raised interest as antiangiogenic compound and is currently used in oncology and cardiology. Therefore we investigated the effects of pac...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta ophthalmologica (Oxford, England) England), 2014-09, Vol.92 (s253), p.0-0
Hauptverfasser: JASIELSKA, M, KOCIOK, N, BRäUTIGAM, M, JOUSSEN, M, PLEYER, U, MAIER, AK
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 0
container_issue s253
container_start_page 0
container_title Acta ophthalmologica (Oxford, England)
container_volume 92
creator JASIELSKA, M
KOCIOK, N
BRäUTIGAM, M
JOUSSEN, M
PLEYER, U
MAIER, AK
description Purpose Paclitaxel is a cytoskeletal agent that interacts with mitotic activity and inhibits microtuble disassembly. Consequently its antiproliferative effect has raised interest as antiangiogenic compound and is currently used in oncology and cardiology. Therefore we investigated the effects of paclitaxel on the angiogenesis and lymphangiogenesis process in a murine corneal neovascularization model. Methods The mouse model of suture‐induced corneal neovascularization was used to assess the anti‐hemangiogenic and anti‐lymphangiogenic effect of paclitaxel by topical application, paclitaxel coated suture. As positive control we used dexamethason eye drops and as negative control – albumin eye drops. Application of eye drops was performed (3x/day) for 2 weeks beginning at the day of the suture‐treatment. Corneal neovascularization formation (angiogenesis and lymphangiogenesis) was quantified on corneal flatmounts stained with CD31 and LYVE‐1. Results Topical applications of paclitaxel significantly inhibited the outgrowth of lymphatic vessels. In contrast, we did not detect an anti‐angiogenic effect of paclitaxel on corneal neovascularization, although the extend of corneal neovascularization was significantly reduced in animals treated with dexamethason (positive control). Conclusion Topical application of paclitaxel inhibits lymphangiogenesis in the cornea. This finding suggests a potential role of paclitaxel in the treatment of corneal diseases.
doi_str_mv 10.1111/j.1755-3768.2014.S074.x
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1554654842</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3408349951</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1294-df72d3272f0eb75e9e9dd041b7b0422a588755e13f64e2979c468d77b9afe1bd3</originalsourceid><addsrcrecordid>eNqNkMtqwzAQRUVpoWnab6iga7uSrIcN3YTQFwSySArdCdkeJTaO5UoOTf6-NimB7jqbeTD3znAQuqckpkM81jFVQkSJkmnMCOXxiigeHy7Q5Dy_PNfi8xrdhFATIqmUfIJW6y1gsBaKPmBncWeKpurNARrsWmzaTeU20EKowtCUuDnuuu2fadXifrDYuX0AXDjfgrlFV9Y0Ae5-8xR9vDyv52_RYvn6Pp8tooKyjEelVaxMmGKWQK4EZJCVJeE0VznhjBmRpsPTQBMrObBMZQWXaalUnhkLNC-TKXo4-Xbefe0h9Lp2e98OJzUVgkvBU86GLXXaKrwLwYPVna92xh81JXokqGs90tEjKT0S1CNBfRiUTyfld9XA8b8yPVuuxir5AZIud7c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1554654842</pqid></control><display><type>article</type><title>The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea</title><source>Access via Wiley Online Library</source><source>Wiley Online Library (Open Access Collection)</source><creator>JASIELSKA, M ; KOCIOK, N ; BRäUTIGAM, M ; JOUSSEN, M ; PLEYER, U ; MAIER, AK</creator><creatorcontrib>JASIELSKA, M ; KOCIOK, N ; BRäUTIGAM, M ; JOUSSEN, M ; PLEYER, U ; MAIER, AK</creatorcontrib><description>Purpose Paclitaxel is a cytoskeletal agent that interacts with mitotic activity and inhibits microtuble disassembly. Consequently its antiproliferative effect has raised interest as antiangiogenic compound and is currently used in oncology and cardiology. Therefore we investigated the effects of paclitaxel on the angiogenesis and lymphangiogenesis process in a murine corneal neovascularization model. Methods The mouse model of suture‐induced corneal neovascularization was used to assess the anti‐hemangiogenic and anti‐lymphangiogenic effect of paclitaxel by topical application, paclitaxel coated suture. As positive control we used dexamethason eye drops and as negative control – albumin eye drops. Application of eye drops was performed (3x/day) for 2 weeks beginning at the day of the suture‐treatment. Corneal neovascularization formation (angiogenesis and lymphangiogenesis) was quantified on corneal flatmounts stained with CD31 and LYVE‐1. Results Topical applications of paclitaxel significantly inhibited the outgrowth of lymphatic vessels. In contrast, we did not detect an anti‐angiogenic effect of paclitaxel on corneal neovascularization, although the extend of corneal neovascularization was significantly reduced in animals treated with dexamethason (positive control). Conclusion Topical application of paclitaxel inhibits lymphangiogenesis in the cornea. This finding suggests a potential role of paclitaxel in the treatment of corneal diseases.</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/j.1755-3768.2014.S074.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Angiogenesis ; Medical treatment ; Ophthalmology ; Rodents</subject><ispartof>Acta ophthalmologica (Oxford, England), 2014-09, Vol.92 (s253), p.0-0</ispartof><rights>2014 Acta Ophthalmologica</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1755-3768.2014.S074.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45575,46833</link.rule.ids></links><search><creatorcontrib>JASIELSKA, M</creatorcontrib><creatorcontrib>KOCIOK, N</creatorcontrib><creatorcontrib>BRäUTIGAM, M</creatorcontrib><creatorcontrib>JOUSSEN, M</creatorcontrib><creatorcontrib>PLEYER, U</creatorcontrib><creatorcontrib>MAIER, AK</creatorcontrib><title>The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea</title><title>Acta ophthalmologica (Oxford, England)</title><description>Purpose Paclitaxel is a cytoskeletal agent that interacts with mitotic activity and inhibits microtuble disassembly. Consequently its antiproliferative effect has raised interest as antiangiogenic compound and is currently used in oncology and cardiology. Therefore we investigated the effects of paclitaxel on the angiogenesis and lymphangiogenesis process in a murine corneal neovascularization model. Methods The mouse model of suture‐induced corneal neovascularization was used to assess the anti‐hemangiogenic and anti‐lymphangiogenic effect of paclitaxel by topical application, paclitaxel coated suture. As positive control we used dexamethason eye drops and as negative control – albumin eye drops. Application of eye drops was performed (3x/day) for 2 weeks beginning at the day of the suture‐treatment. Corneal neovascularization formation (angiogenesis and lymphangiogenesis) was quantified on corneal flatmounts stained with CD31 and LYVE‐1. Results Topical applications of paclitaxel significantly inhibited the outgrowth of lymphatic vessels. In contrast, we did not detect an anti‐angiogenic effect of paclitaxel on corneal neovascularization, although the extend of corneal neovascularization was significantly reduced in animals treated with dexamethason (positive control). Conclusion Topical application of paclitaxel inhibits lymphangiogenesis in the cornea. This finding suggests a potential role of paclitaxel in the treatment of corneal diseases.</description><subject>Angiogenesis</subject><subject>Medical treatment</subject><subject>Ophthalmology</subject><subject>Rodents</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkMtqwzAQRUVpoWnab6iga7uSrIcN3YTQFwSySArdCdkeJTaO5UoOTf6-NimB7jqbeTD3znAQuqckpkM81jFVQkSJkmnMCOXxiigeHy7Q5Dy_PNfi8xrdhFATIqmUfIJW6y1gsBaKPmBncWeKpurNARrsWmzaTeU20EKowtCUuDnuuu2fadXifrDYuX0AXDjfgrlFV9Y0Ae5-8xR9vDyv52_RYvn6Pp8tooKyjEelVaxMmGKWQK4EZJCVJeE0VznhjBmRpsPTQBMrObBMZQWXaalUnhkLNC-TKXo4-Xbefe0h9Lp2e98OJzUVgkvBU86GLXXaKrwLwYPVna92xh81JXokqGs90tEjKT0S1CNBfRiUTyfld9XA8b8yPVuuxir5AZIud7c</recordid><startdate>201409</startdate><enddate>201409</enddate><creator>JASIELSKA, M</creator><creator>KOCIOK, N</creator><creator>BRäUTIGAM, M</creator><creator>JOUSSEN, M</creator><creator>PLEYER, U</creator><creator>MAIER, AK</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>201409</creationdate><title>The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea</title><author>JASIELSKA, M ; KOCIOK, N ; BRäUTIGAM, M ; JOUSSEN, M ; PLEYER, U ; MAIER, AK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1294-df72d3272f0eb75e9e9dd041b7b0422a588755e13f64e2979c468d77b9afe1bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Angiogenesis</topic><topic>Medical treatment</topic><topic>Ophthalmology</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JASIELSKA, M</creatorcontrib><creatorcontrib>KOCIOK, N</creatorcontrib><creatorcontrib>BRäUTIGAM, M</creatorcontrib><creatorcontrib>JOUSSEN, M</creatorcontrib><creatorcontrib>PLEYER, U</creatorcontrib><creatorcontrib>MAIER, AK</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JASIELSKA, M</au><au>KOCIOK, N</au><au>BRäUTIGAM, M</au><au>JOUSSEN, M</au><au>PLEYER, U</au><au>MAIER, AK</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><date>2014-09</date><risdate>2014</risdate><volume>92</volume><issue>s253</issue><spage>0</spage><epage>0</epage><pages>0-0</pages><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Purpose Paclitaxel is a cytoskeletal agent that interacts with mitotic activity and inhibits microtuble disassembly. Consequently its antiproliferative effect has raised interest as antiangiogenic compound and is currently used in oncology and cardiology. Therefore we investigated the effects of paclitaxel on the angiogenesis and lymphangiogenesis process in a murine corneal neovascularization model. Methods The mouse model of suture‐induced corneal neovascularization was used to assess the anti‐hemangiogenic and anti‐lymphangiogenic effect of paclitaxel by topical application, paclitaxel coated suture. As positive control we used dexamethason eye drops and as negative control – albumin eye drops. Application of eye drops was performed (3x/day) for 2 weeks beginning at the day of the suture‐treatment. Corneal neovascularization formation (angiogenesis and lymphangiogenesis) was quantified on corneal flatmounts stained with CD31 and LYVE‐1. Results Topical applications of paclitaxel significantly inhibited the outgrowth of lymphatic vessels. In contrast, we did not detect an anti‐angiogenic effect of paclitaxel on corneal neovascularization, although the extend of corneal neovascularization was significantly reduced in animals treated with dexamethason (positive control). Conclusion Topical application of paclitaxel inhibits lymphangiogenesis in the cornea. This finding suggests a potential role of paclitaxel in the treatment of corneal diseases.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1755-3768.2014.S074.x</doi><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1755-375X
ispartof Acta ophthalmologica (Oxford, England), 2014-09, Vol.92 (s253), p.0-0
issn 1755-375X
1755-3768
language eng
recordid cdi_proquest_journals_1554654842
source Access via Wiley Online Library; Wiley Online Library (Open Access Collection)
subjects Angiogenesis
Medical treatment
Ophthalmology
Rodents
title The effects of paclitaxel on angiogenesis and lymphangiogenesis in the mouse cornea
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T04%3A09%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effects%20of%20paclitaxel%20on%20angiogenesis%20and%20lymphangiogenesis%20in%20the%20mouse%20cornea&rft.jtitle=Acta%20ophthalmologica%20(Oxford,%20England)&rft.au=JASIELSKA,%20M&rft.date=2014-09&rft.volume=92&rft.issue=s253&rft.spage=0&rft.epage=0&rft.pages=0-0&rft.issn=1755-375X&rft.eissn=1755-3768&rft_id=info:doi/10.1111/j.1755-3768.2014.S074.x&rft_dat=%3Cproquest_cross%3E3408349951%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1554654842&rft_id=info:pmid/&rfr_iscdi=true