Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection
Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycopro...
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| Veröffentlicht in: | Vaccine 2005-07, Vol.23 (34), p.4342 |
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| creator | González, Belén Reina, Ramsés García, Iker Andrés, Sara Glaria, Idoia Alzueta, María Mora, María Isabel Jugo, Begoña M Arrieta-Aguirre, Inés de la Lastra, José M Pérez Rodríguez, Dolores Rodríguez, Juan Ramón Esteban, Mariano Grilló, María Jesús Blacklaws, Barbara A Harkiss, Gordon D Chebloune, Yahia Luján, Lluís de Andrés, Damián Amorena, Beatriz |
| description | Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-envand pCR3.1-IFN-γplasmid inoculations. The pcDNA plasmid used in the control group contained thelacZcoding sequences instead of theenvgene. Within a month post-challenge, the viral load in the vaccinated group was lower (p |
| doi_str_mv | 10.1016/j.vaccine.2005.03.032 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_1547059847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3379189901</sourcerecordid><originalsourceid>FETCH-proquest_journals_15470598473</originalsourceid><addsrcrecordid>eNqNzc1KxDAUBeAgCtafRxAuuNFFa9KfaWcpOuKmrqS4Gy6Z1Lmlk4y9ScV5eiPMAwgHzuJ8cIS4UTJTUi0ehmxGrcmaLJeyymQRk5-IRDV1keaVak5FIvNFmZZKfpyLC-ZBRlioZSIObdCOcQTa7YKlA3pyFlwPvDVmD9_kt4DQotlQ2hFbhJmmwHDXdt29sfPz2yMc32E_OW-0Z8BPJMseDE7jD0T5N22C9jQbINtHFF-uxFmPI5vrY1-K25fV-9NrGvFXMOzXgwuTjdNaVWUtq2VT1sX_1C_xI1b4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1547059847</pqid></control><display><type>article</type><title>Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection</title><source>Elsevier ScienceDirect Journals Complete</source><source>ProQuest Central UK/Ireland</source><creator>González, Belén ; Reina, Ramsés ; García, Iker ; Andrés, Sara ; Glaria, Idoia ; Alzueta, María ; Mora, María Isabel ; Jugo, Begoña M ; Arrieta-Aguirre, Inés ; de la Lastra, José M Pérez ; Rodríguez, Dolores ; Rodríguez, Juan Ramón ; Esteban, Mariano ; Grilló, María Jesús ; Blacklaws, Barbara A ; Harkiss, Gordon D ; Chebloune, Yahia ; Luján, Lluís ; de Andrés, Damián ; Amorena, Beatriz</creator><creatorcontrib>González, Belén ; Reina, Ramsés ; García, Iker ; Andrés, Sara ; Glaria, Idoia ; Alzueta, María ; Mora, María Isabel ; Jugo, Begoña M ; Arrieta-Aguirre, Inés ; de la Lastra, José M Pérez ; Rodríguez, Dolores ; Rodríguez, Juan Ramón ; Esteban, Mariano ; Grilló, María Jesús ; Blacklaws, Barbara A ; Harkiss, Gordon D ; Chebloune, Yahia ; Luján, Lluís ; de Andrés, Damián ; Amorena, Beatriz</creatorcontrib><description>Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-envand pCR3.1-IFN-γplasmid inoculations. The pcDNA plasmid used in the control group contained thelacZcoding sequences instead of theenvgene. Within a month post-challenge, the viral load in the vaccinated group was lower (p<=0.05) and virus was only detected transiently compared with the control group. Furthermore, 2 months later, neutralizing antibodies (NtAb) were detected in all the control animals and none of the vaccinated animals (p<=0.01). These results demonstrated a significant early protective effect of this immunization strategy against MVV infection that restricts the virus replication following challenge in the absence of NtAb production. This vaccine protective effect against MVV infection disappeared after two years post-challenge, when active replication of MVV challenge strain was observed. Protection conferred by the vaccine could not be explained byOLA DRB1allele or genotype differences. Most of the individuals wereDRB1heterozygous and none was totally resistant to infection.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.03.032</identifier><language>eng</language><publisher>Kidlington: Elsevier Limited</publisher><subject>Antigens ; Binding sites ; Deoxyribonucleic acid ; DNA ; Experiments ; Genes ; Glycoproteins ; Immune system ; Immunization ; Infections ; Plasmids ; Sheep ; Vaccines</subject><ispartof>Vaccine, 2005-07, Vol.23 (34), p.4342</ispartof><rights>Copyright Elsevier Limited Jul 29, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1547059847?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>González, Belén</creatorcontrib><creatorcontrib>Reina, Ramsés</creatorcontrib><creatorcontrib>García, Iker</creatorcontrib><creatorcontrib>Andrés, Sara</creatorcontrib><creatorcontrib>Glaria, Idoia</creatorcontrib><creatorcontrib>Alzueta, María</creatorcontrib><creatorcontrib>Mora, María Isabel</creatorcontrib><creatorcontrib>Jugo, Begoña M</creatorcontrib><creatorcontrib>Arrieta-Aguirre, Inés</creatorcontrib><creatorcontrib>de la Lastra, José M Pérez</creatorcontrib><creatorcontrib>Rodríguez, Dolores</creatorcontrib><creatorcontrib>Rodríguez, Juan Ramón</creatorcontrib><creatorcontrib>Esteban, Mariano</creatorcontrib><creatorcontrib>Grilló, María Jesús</creatorcontrib><creatorcontrib>Blacklaws, Barbara A</creatorcontrib><creatorcontrib>Harkiss, Gordon D</creatorcontrib><creatorcontrib>Chebloune, Yahia</creatorcontrib><creatorcontrib>Luján, Lluís</creatorcontrib><creatorcontrib>de Andrés, Damián</creatorcontrib><creatorcontrib>Amorena, Beatriz</creatorcontrib><title>Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection</title><title>Vaccine</title><description>Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-envand pCR3.1-IFN-γplasmid inoculations. The pcDNA plasmid used in the control group contained thelacZcoding sequences instead of theenvgene. Within a month post-challenge, the viral load in the vaccinated group was lower (p<=0.05) and virus was only detected transiently compared with the control group. Furthermore, 2 months later, neutralizing antibodies (NtAb) were detected in all the control animals and none of the vaccinated animals (p<=0.01). These results demonstrated a significant early protective effect of this immunization strategy against MVV infection that restricts the virus replication following challenge in the absence of NtAb production. This vaccine protective effect against MVV infection disappeared after two years post-challenge, when active replication of MVV challenge strain was observed. Protection conferred by the vaccine could not be explained byOLA DRB1allele or genotype differences. Most of the individuals wereDRB1heterozygous and none was totally resistant to infection.</description><subject>Antigens</subject><subject>Binding sites</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Experiments</subject><subject>Genes</subject><subject>Glycoproteins</subject><subject>Immune 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infection</atitle><jtitle>Vaccine</jtitle><date>2005-07-29</date><risdate>2005</risdate><volume>23</volume><issue>34</issue><spage>4342</spage><pages>4342-</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-envand pCR3.1-IFN-γplasmid inoculations. The pcDNA plasmid used in the control group contained thelacZcoding sequences instead of theenvgene. Within a month post-challenge, the viral load in the vaccinated group was lower (p<=0.05) and virus was only detected transiently compared with the control group. Furthermore, 2 months later, neutralizing antibodies (NtAb) were detected in all the control animals and none of the vaccinated animals (p<=0.01). These results demonstrated a significant early protective effect of this immunization strategy against MVV infection that restricts the virus replication following challenge in the absence of NtAb production. This vaccine protective effect against MVV infection disappeared after two years post-challenge, when active replication of MVV challenge strain was observed. Protection conferred by the vaccine could not be explained byOLA DRB1allele or genotype differences. Most of the individuals wereDRB1heterozygous and none was totally resistant to infection.</abstract><cop>Kidlington</cop><pub>Elsevier Limited</pub><doi>10.1016/j.vaccine.2005.03.032</doi></addata></record> |
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| ispartof | Vaccine, 2005-07, Vol.23 (34), p.4342 |
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| source | Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Antigens Binding sites Deoxyribonucleic acid DNA Experiments Genes Glycoproteins Immune system Immunization Infections Plasmids Sheep Vaccines |
| title | Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection |
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