Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection

Mucosal immunization of sheep with a Maedi-Visna virus (MVV)envDNA vaccine protects against early MVV productive infection

Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycopro...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Vaccine 2005-07, Vol.23 (34), p.4342
Hauptverfasser: González, Belén, Reina, Ramsés, García, Iker, Andrés, Sara, Glaria, Idoia, Alzueta, María, Mora, María Isabel, Jugo, Begoña M, Arrieta-Aguirre, Inés, de la Lastra, José M Pérez, Rodríguez, Dolores, Rodríguez, Juan Ramón, Esteban, Mariano, Grilló, María Jesús, Blacklaws, Barbara A, Harkiss, Gordon D, Chebloune, Yahia, Luján, Lluís, de Andrés, Damián, Amorena, Beatriz
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Binding sites
Deoxyribonucleic acid
DNA
Experiments
Genes
Glycoproteins
Immune system
Immunization
Infections
Plasmids
Sheep
Vaccines
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Gene gun mucosal DNA immunization of sheep with a plasmid expressing theenvgene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-envand pCR3.1-IFN-γplasmid inoculations. The pcDNA plasmid used in the control group contained thelacZcoding sequences instead of theenvgene. Within a month post-challenge, the viral load in the vaccinated group was lower (p
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2005.03.032