Photo-Cross-Linkers Incorporated into G-Protein-Coupled Receptors in Mammalian Cells: A Ligand Comparison

Capturing the right ligand at the right spot: A well‐balanced system for non‐natural amino acid mutagenesis allows the ligand binding sites of a class II G‐protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian...

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Veröffentlicht in:	Angewandte Chemie International Edition 2011-08, Vol.50 (35), p.8077-8081
Hauptverfasser:	Coin, Irene, Perrin, Marilyn H., Vale, Wylie W., Wang, Lei
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - chemistry Animals Binding Sites genetic code HEK293 Cells Humans Ligands Mutagenesis, Site-Directed non-natural amino acids photo-cross-linking photoaffinity labeling Photoaffinity Labels Rats Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism
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Zusammenfassung:	Capturing the right ligand at the right spot: A well‐balanced system for non‐natural amino acid mutagenesis allows the ligand binding sites of a class II G‐protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201102646