Cloning and Characterization of Walnut Lipid Transfer Protein: Not a Major Allergen in Walnut-Allergic U.S. Patients

RNA was isolated from walnut embryos and used as a template for cDNA. 3' and 5' RACE was performed using primers based on the coding sequence for walnut lipid transfer protein from a walnut embryo EST database.

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Veröffentlicht in:Journal of allergy and clinical immunology 2006-02, Vol.117 (2), p.S43-S43
Hauptverfasser: Teuber, S.S., Pastorello, E., Peterson, W.R., Dandekar, A.M., Uratsu, S.
Format: Artikel
Sprache:eng
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Zusammenfassung:RNA was isolated from walnut embryos and used as a template for cDNA. 3' and 5' RACE was performed using primers based on the coding sequence for walnut lipid transfer protein from a walnut embryo EST database.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2005.12.178