Gene and Protein Expression Analysis of Extra-Cellular Matrix Proteins During Initiation and Resolution of Asthma in a Mouse Model

To examine this process, we followed the global expression of relevant extracellular matrix components during initiation and resolution of allergic inflammation in a well-characterized aspergillus asthma model (Haczku, et al, Am J Respir Cell Mol Biol. 2001,25:45-50) Methods Multiple gene expression was determined by real-time PCR in whole lung lysates at baseline, and 1, 7 and 10 days post nasal allergen challenge in sensitized mice.