Prostaglandin E2 Promotes Net Plasminogen Activation in Human Lung Fibroblasts

IMR-90 human fetal fibroblasts were incubated for 24 and 48 hours with serum-free medium in the presence or absence of PGE2 at concentrations ranging from 10 nM to 1000 nM. uPA and PAI-1 protein secreted into the medium was determined by immunoblot analysis, and net uPA activity in medium by alpha-c...

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Veröffentlicht in:	Journal of allergy and clinical immunology 2009-02, Vol.123 (2), p.S120-S120
Hauptverfasser:	Kovalszki, A, Wettlaufer, S, Huang, S, Sisson, T.H, Peters-Golden, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology
Online-Zugang:	Volltext
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Zusammenfassung:	IMR-90 human fetal fibroblasts were incubated for 24 and 48 hours with serum-free medium in the presence or absence of PGE2 at concentrations ranging from 10 nM to 1000 nM. uPA and PAI-1 protein secreted into the medium was determined by immunoblot analysis, and net uPA activity in medium by alpha-casein zymography.
ISSN:	0091-6749 1097-6825
DOI:	10.1016/j.jaci.2008.12.441