Establishing a minimum incubation time for biological indicators

For exposed Bis that have only one surviving spore, one of two conditions can exist: the spore has not been damaged in a manner that significantly affects either germination time or post-germination growth rate, or damage has occurred that significantly affects one or both of these characteristics, when a sterilisation exposure gives a quantal-zone result of 50% of the Bis testing nonsterile, the Poisson distribution predicts approximately 35 Bis will have only one surviving spore and the remaining 15 will...