MYELOPEROXIDASE AND GLUTAMATE-CYSTEINE LIGASE POLYMORPHISMS IN TYPE 2 DIABETES MELLITUS: A PRELIMINARY STUDY / POLIMORFIZMI MIJELOPEROKSIDAZE I GLUTAMAT-CISTEIN LIGAZE U DIABETES MELLITUSU TIPA 2: PRELIMINARNA STUDIJA

Background: Oxidative stress can accompany the physio- pathology of diabetes mellitus. Myelopercecidase and gluta- mate-cysteine ligase catalytic subunit are two important sub- stances which are connected with the maintaining of the redox balance in the body. In this study, the association be- tween diabetes, lipid levels, myeloperoxidase -463G/A, and the glutamate-cysteine ligase catalytic subunit -3506A/G gene polymorphisms have been investigated. Methods: Frequencies of genotypes and alleles have been assessed using PCR-RFLP techniques in 90 type 2 diabetic patients and 70 healthy controls. Results: We found an association between myeloperoxidase -463 G/A genotype distributions (p=0.023) and allele distri- butions (p=0.009) in the study groups. In addition, there is a significant difference for each of the genotype (p=0.014) and allele (p=0.007) distributions for the glutamate-cysteine ligase catalytic subunit -3506A/G polymorphism. Conclusions: Our study reveals the first data about the myeloperoxidase -463G/A and glutamate-cysteine ligase catalytic subunit -3506A/G gene polymorphisms for type 2 diabetes and we believe that this work will play a pioneering role for further studies. Uvod: Fiziopatologiju diabetes mellitusa može pratiti oksida- trvni stres. Katalitičke podjedinice mijeloperoksidaze i gluta- mat-cistein ligaze su dve važne supstance koje su povezane sa održavanjem redoks ravnoteže u telu. U ovoj studiji, ispi- tivana je veza između dijabetesa, n'rvoa lipida i genskih poli- morfizama -463G/A mijeloperoksidaze i -3506A/G katali- tičke podjedinice glutamat-cistein ligaze. Metode: Učestalost genotipova i alela određena je pomoću tehnika PCR-RFLP kod 90 pacijenata sa dijabetesom tipa 2 i 70 zdravih kontrolnih subjekata. Rezultati: Otkrili smo povezanost između distribucija ge- notipa (p=0,023) i distribucija alela (p=0,009) -463 G/A mijeloperoksidaze u proučavanim grupama. Pored toga, postoji značajna razlika za svaku od distribucija genotipova (р=0,014) i alela (p=0,007) za polimorfizam katalitičke podjedinice -3506A/G glutamat-cistein ligaze. Zaključak: ^laša studija otkriva prve podatke o genskim poli- morfizmima -463G/A mijebperoksidaze i -3506A/G katali- tičke podjedinice glutamat-cistein ligaze za dijabetes tipa 2 i verujemo da će ovaj rad imati pionirsku ulogu za buduće studije.