Biological Activity of Clothianidin against Anomala cuprea (Hope) (Coleoptera

The insecticidal activity of clothianidin, a neonicotinoid insecticide, against Anomala cuprea (Hope) was investigated. The LC50 values of clothianidin for the 1st and 2nd instar larvae at 14 days after treatment were 0.0075 mg a.i./kg soil and 0.21 mg a.i./kg soil, respectively. The LC50 for the 3rd instar larvae was not determined, but was estimated to be between 0.30 and 3.0 mg a.i./kg soil. Clothianidin showed antifeeding and growth inhibition activities against 3rd instar larvae at 0.10 mg a.i./kg soil. Furthermore, clothianidin suppressed the crawling movement of 3rd instar larvae at 0.50 mg a.i./kg soil. These properties of clothianidin would be useful to establish the controlling methods of A. cuprea.