Simplified techniques for determination of polyploidy in Ginbuna Carassius auratus langsdorfi [carp]

In order to contribute to the studies on ginbuna Carassius auratus langsdorfi, some attempts were made to develop a convenient method for determination of polyploidy, using 62 ginbuna specimens collected from 12 localities in Japan and one in Korea. By karyotype analysis, it was disclosed that they were composed of 17 diploids and 45 triploids. Then a variety of tissue proteins and enzymes of those fishes were analyzed electrophoretically on starch gel and the electropherograms obtained were compared to look for any significant difference between the diploids and triploids. Results showed that myogen and creatine kinase are useful markers for determining di- and triploidy, as far as the specimens collected from localities other than Lake Kasumigaura were concerned. The determination method which is based on the differences in their erythrocytic sizes was also satisfactory. The specimens collected from Lake Kasumigaura were all found to be triploid by karyotype analysis but some of them were erroneously determined to be diploids by the simplified techniques.