High Speed Liquid Chromatography Using Spherical Porous Copolymer of Styrene-Divinylbenzene

Spherical porous copolymer of styrene-divinylbenzene was packed in high speed liquid chromatography column and separation parameters of the column eilficiency, solvent composition of mobile phase, column temperature and sample loading capacity has been studied. Since the porous polymer has specific surface area of 328 m2/g, its sample loading capacity is greater than that of pellicular type packings. The porous polymer with particle size of 25 2ptm exhibited the column efliciency (HETP) of l mm, when 11-deoxycorticosterone was separated at l cm/sec of carrier velocity. High efficiency sepaparation was obtained by using methanol as a mobile phase. lt was possible to reduce the retention volume and improve the column eMciency by adding n-hexane to the methanol mobile phase. Resolution was improved by the addition of water to the methanol mobile phase, altheugh the retention volume increased, Using the porous polymer, rapid separation of amine, heterocyclic compounds, steroid hormones are lpossible. For example, using 500 mm long and 2. l mm i. d. column of 53CC, estriol, ethYnylestradiol and estrone were separated within 12 minutes by eluting them by 15 volO/o of H2Q in methanel at the rate of O.82 ml/min.