Detection of alien genes and analysis of their integration position in transgenic rice by fluorescence In situ hybridization

Using fluorescence in situ hybridization (FISH), we localized transferred barnase-ps1 and pHctinG DNA sequences onto chromosomes in a group of rice transgenic plants cotransformed by microprojectile bombardment. In all of the tested rice transgenic lines, the detected cells showed 1-3 signal spots and they were located in the central and terminal regions of chromosome arms. The signals of both barnase-ps1 and pHctinG genes were mostly detected in close contact on hromosomes, which could not be resolved without using two-color in situ hybridization. In a few cells, the signals were separated from each other for barnase-ps1 and pHctinG.