Angiotensin I-Converting Enzyme Inhibitors in Fish Water Soluble Protein Hydrolyzates Prepared by Bioreactor

Liquid seasoning was prepared from the wash water produced during cod surimi production, which is normally discarded, using an hydrolysis test plant. This solution had not only a good flavor but also showed inhibitory activity on the angiotensin I-converting enzyme (ACE). Inhibitors in the liquid seasoning showed a molecular weight distribution of about 200 to 500 by gel filtration chromatography. Two inhibitory peptides were isolated from the liquid seasoning using reverse-phase HPLC. The amino acid sequences of inhibitory peptides were Val-Trp and Ile-Trp. Peptide sequences of Val-Trp and Ile-Trp were found in the primary structure of actin. Val-Trp fragment exists also in myoglobin.