Covalent Immobilization of Metal-binding Motifs of Enzymes on Quartz Surface. [Ni(Cys-X2-Cys)2]2− of Hydrogenases

Covalent Immobilization of Metal-binding Motifs of Enzymes on Quartz Surface. [Ni(Cys-X2-Cys)2]2− of Hydrogenases

The peptide ligand, Cys-Pro-Leu-Cys, was covalently immobilized on the surface of quartz via (3-aminopropyl) triethoxysilane (APTES) by the general peptide-synthesis method; then, Ni2+ was introduced into this surface to construct the [Ni(S-Cys)4]2− unit contained in the active site of hydrogenases....

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Veröffentlicht in:Chemistry letters 2005-12, Vol.34 (12), p.1634-1635
Hauptverfasser: Sakaniwa, Daisuke, Ohe, Takahiro, Misumi, Takashi, Monjushiro, Hideaki, Onoda, Akira, Yamamura, Takeshi
Format: Artikel
Sprache:eng
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Zusammenfassung:The peptide ligand, Cys-Pro-Leu-Cys, was covalently immobilized on the surface of quartz via (3-aminopropyl) triethoxysilane (APTES) by the general peptide-synthesis method; then, Ni2+ was introduced into this surface to construct the [Ni(S-Cys)4]2− unit contained in the active site of hydrogenases.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2005.1634