Problem‐solving test: Conditional gene targeting using the Cre/loxP recombination system

Terms to be familiar with before you start to solve the test: gene targeting, knock‐out mutation, bacteriophage, complementary base‐pairing, homologous recombination, deletion, transgenic organisms, promoter, polyadenylation element, transgene, DNA replication, RNA polymerase, Shine‐Dalgarno sequence, restriction endonuclease, polymerase chain reaction, primer, transcription, SV40 virus, malignant transformation, spacer region, gene expression, agarose gel electrophoresis, ethidium bromide staining, Southern‐blotting, probe, reporter gene. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(6):445–449, 2013