Cloning and Expression of a [beta]-Galactosidase Gene of Bacillus circulans

Cloning and Expression of a [beta]-Galactosidase Gene of Bacillus circulans

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011-06, Vol.75 (6), p.1194
Hauptverfasser: SONG, Jingyuan, IMANAKA, Hiroyuki, IMAMURA, Koreyoshi, MINODA, Masashi, KATASE, Toru, HOSHI, Yukiko, YAMAGUCHI, Shotaro, NAKANISHI, Kazuhiro
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Sprache:eng
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Zusammenfassung:A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
ISSN:0916-8451
1347-6947