Determination of Branched [beta]-Cyclodextrin-Prostaglandin Complexes Using Electrospray Ionization Mass Spectrometry

Mass spectral measurements by electrospray ionization mass spectrometry (ESI-MS) detected the ions of β-cyclodextrin (βCD) or branched βCDs (glucosyl-, galactosyl-, mannosyl- and maltosyl-βCD)-prostaglandins (PGs: PGA2, PGD2, PGE1, PGE2, PGF2α and PGJ2) complexes, i.e., βCD-PG complexes, with a host:guest ratio of 1:1 in the negative ion mode. This is the first study to report the ions of branched βCD-PG complexes using ESI-MS. The inclusion complexes were determined by a flow injection analysis using acetonitrile/water. We could confirm by this method the presence of a βCD-PGE2 complex with a host:guest ratio of 1:1 in a solution-dissolved pharmaceutical formulation consisting of βCD-PGE2 (ProstarmonTM E tablet).