Detection of walnut [Juglans regia] residues in processed foods by polymerase chain reaction

Detection of walnut [Juglans regia] residues in processed foods by polymerase chain reaction

A sensitive qualitative detection method for walnut (Juglans regia) using polymerase chain reaction (PCR) was developed. For detection of walnuts with high specificity, the primer pair WAL-F/WAL-R was designed based on walnut matK genes. Trace amounts of walnuts in commercial food products can be qu...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-07, Vol.71 (7), p.1793-1796
Hauptverfasser: Yano, T.(Oriental Yeast Co. Ltd., Nagahama, Shiga (Japan). Nagahama Inst. for Biochemical Science), Sakai, Y, Uchida, K, Nakao, Y, Ishihata, K, Nakano, S, Yamada, T, Sakai, S, Urisu, A, Akiyama, H, Maitani, T
Format: Artikel
Sprache:eng
Schlagworte:
ALERGENOS
allergen
ALLERGENE
ALLERGENS
Antigens, Plant - analysis
Antigens, Plant - genetics
Base Sequence
Biological and medical sciences
Food Analysis
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genome, Plant
Juglans - genetics
JUGLANS REGIA
Molecular Sequence Data
NOIX
NUEZ
PCR
pecan nut
Polymerase Chain Reaction
restriction enzyme
walnut
WALNUTS
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Beschreibung
Zusammenfassung:A sensitive qualitative detection method for walnut (Juglans regia) using polymerase chain reaction (PCR) was developed. For detection of walnuts with high specificity, the primer pair WAL-F/WAL-R was designed based on walnut matK genes. Trace amounts of walnuts in commercial food products can be qualitatively detected using this method.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.70118