Stimulation of mycelia growth in several mushroom species by rice husks

When supplemented to the culture medium of mushroom Coprinus cinereus, rice husks soaked beforehand In methanol stimulated mycelia growth up to a concentration of 80mg/ml dose-dependently, whereas the non- treated stimulated mycelia growth up to 20mg/ml. This result suggests the existence of both stimulatory and inhibitory substances in rice husks. Since momilactone A (MLA) is recognized as one of the phytoalexins in rice husks, its biological activity against mycelia growth was tested. Momilactone A inhibited myceila growth at 5 Microg/disc, whereas the methanol extract of husks did so at 1 mg/disc, wherein 0.2 Microg of MLA was estimated by LC/MS/MS. Thus the phytoalexins including MLA should inhibit mycelia growth. Rice husks stimulated mycelia growth in some edible mushroom species such as Gnfola frondosa (maitake), Lentinus edodes (shiitake), Pleurotus eryngii (eringi), and P. ostreatus (hiratake). Our findings might lead to the development of new profitable cultivation methods for mushrooms using rice husks.