Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein
Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293 rd position did not restore cell growth of E. coli CF8295 (Δ relA, Δ spoT) and did not accumulate ppGpp in the cell, sug...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-12, Vol.66 (12), p.2735-2738 |
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| container_issue | 12 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 66 |
| creator | Fujita, C. (Nara Women's Univ. (Japan). Faculty of Science) Maeda, M Fujii, T Iwamoto, R Ikehara, K |
| description | Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293
rd
position did not restore cell growth of E. coli CF8295 (Δ
relA, Δ
spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein. |
| doi_str_mv | 10.1271/bbb.66.2735 |
| format | Article |
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rd
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relA, Δ
spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.66.2735</identifier><identifier>PMID: 12596879</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Alanine - genetics ; Amino Acid Sequence ; Amino Acid Substitution ; AMINO ACIDS ; Aspartic Acid - genetics ; Bacteriology ; Biological and medical sciences ; BIOSYNTHESIS ; CATALYTIC ACTIVITY ; catalytic center ; Cell Division ; ESCHERICHIA COLI ; Escherichia coli - enzymology ; Escherichia coli - growth & development ; Escherichia coli - metabolism ; Fundamental and applied biological sciences. Psychology ; Guanosine Tetraphosphate - biosynthesis ; Guanosine Tetraphosphate - metabolism ; IDENTIFICATION ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Mutation ; ppGpp synthesis ; PROTEINS ; Pyrophosphatases - chemistry ; Pyrophosphatases - genetics ; Pyrophosphatases - metabolism ; SpoT protein ; stringent response</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2002-12, Vol.66 (12), p.2735-2738</ispartof><rights>2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-19c230549c00e9013599c49eb358c1d486e9883adab1d5769dfa8d0a4d54573a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14544002$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12596879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujita, C. (Nara Women's Univ. (Japan). Faculty of Science)</creatorcontrib><creatorcontrib>Maeda, M</creatorcontrib><creatorcontrib>Fujii, T</creatorcontrib><creatorcontrib>Iwamoto, R</creatorcontrib><creatorcontrib>Ikehara, K</creatorcontrib><title>Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293
rd
position did not restore cell growth of E. coli CF8295 (Δ
relA, Δ
spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.</description><subject>Alanine - genetics</subject><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution</subject><subject>AMINO ACIDS</subject><subject>Aspartic Acid - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>BIOSYNTHESIS</subject><subject>CATALYTIC ACTIVITY</subject><subject>catalytic center</subject><subject>Cell Division</subject><subject>ESCHERICHIA COLI</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guanosine Tetraphosphate - biosynthesis</subject><subject>Guanosine Tetraphosphate - metabolism</subject><subject>IDENTIFICATION</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>ppGpp synthesis</subject><subject>PROTEINS</subject><subject>Pyrophosphatases - chemistry</subject><subject>Pyrophosphatases - genetics</subject><subject>Pyrophosphatases - metabolism</subject><subject>SpoT protein</subject><subject>stringent response</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhoModrt65bUSEL2RWfM9k8tS2lopKFivw5kk46bMJGMyi-y_N8uuFEQQAoHwvO854UHoFSUbylr6se_7jVIb1nL5BK0oF22jtGifohXRVDWdkPQMnZfyQEh9kPQ5OqNMatW1eoX6W-fjEoZgYQkp4jRgiDhEF8rsY4F-9BimEBMGGxweUsbzfDPPuOzjsvUllEPkqtitz8FuA2CbxoC_zekezzktPsQX6NkAY_EvT_cafb--ur_81Nx9ubm9vLhrrFR6aai2jBMptCXEa0K51NoK7XsuO0ud6JTXXcfBQU-dbJV2A3SOgHBSyJYDX6P3x9469-fOl8VMoVg_jhB92hXTsk4oWov_B1LNmO5aVsG3f4EPaZdj_YShQmghia6T1-jDkbI5lZL9YOYcJsh7Q4k5GDLVkFHKHAxV-s2pc9dP3j2yJyUVeHcCoFgYhwzRhvLICSkEIYfl1JELsVqZ4FfKozML7MeU_4T4vzd4fQwOkAz8yJX7_JURwuuhnPHfmLWy_A</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Fujita, C. 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Faculty of Science) ; Maeda, M ; Fujii, T ; Iwamoto, R ; Ikehara, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-19c230549c00e9013599c49eb358c1d486e9883adab1d5769dfa8d0a4d54573a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Alanine - genetics</topic><topic>Amino Acid Sequence</topic><topic>Amino Acid Substitution</topic><topic>AMINO ACIDS</topic><topic>Aspartic Acid - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>BIOSYNTHESIS</topic><topic>CATALYTIC ACTIVITY</topic><topic>catalytic center</topic><topic>Cell Division</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guanosine Tetraphosphate - biosynthesis</topic><topic>Guanosine Tetraphosphate - metabolism</topic><topic>IDENTIFICATION</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>ppGpp synthesis</topic><topic>PROTEINS</topic><topic>Pyrophosphatases - chemistry</topic><topic>Pyrophosphatases - genetics</topic><topic>Pyrophosphatases - metabolism</topic><topic>SpoT protein</topic><topic>stringent response</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujita, C. (Nara Women's Univ. (Japan). Faculty of Science)</creatorcontrib><creatorcontrib>Maeda, M</creatorcontrib><creatorcontrib>Fujii, T</creatorcontrib><creatorcontrib>Iwamoto, R</creatorcontrib><creatorcontrib>Ikehara, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, C. (Nara Women's Univ. (Japan). 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rd
position did not restore cell growth of E. coli CF8295 (Δ
relA, Δ
spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12596879</pmid><doi>10.1271/bbb.66.2735</doi><tpages>4</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2002-12, Vol.66 (12), p.2735-2738 |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Alanine - genetics Amino Acid Sequence Amino Acid Substitution AMINO ACIDS Aspartic Acid - genetics Bacteriology Biological and medical sciences BIOSYNTHESIS CATALYTIC ACTIVITY catalytic center Cell Division ESCHERICHIA COLI Escherichia coli - enzymology Escherichia coli - growth & development Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Guanosine Tetraphosphate - biosynthesis Guanosine Tetraphosphate - metabolism IDENTIFICATION Metabolism. Enzymes Microbiology Molecular Sequence Data Mutation ppGpp synthesis PROTEINS Pyrophosphatases - chemistry Pyrophosphatases - genetics Pyrophosphatases - metabolism SpoT protein stringent response |
| title | Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein |
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