Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein

Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293 rd position did not restore cell growth of E. coli CF8295 (Δ relA, Δ spoT) and did not accumulate ppGpp in the cell, sug...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-12, Vol.66 (12), p.2735-2738
Hauptverfasser:	Fujita, C. (Nara Women's Univ. (Japan). Faculty of Science), Maeda, M, Fujii, T, Iwamoto, R, Ikehara, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - genetics Amino Acid Sequence Amino Acid Substitution AMINO ACIDS Aspartic Acid - genetics Bacteriology Biological and medical sciences BIOSYNTHESIS CATALYTIC ACTIVITY catalytic center Cell Division ESCHERICHIA COLI Escherichia coli - enzymology Escherichia coli - growth & development Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Guanosine Tetraphosphate - biosynthesis Guanosine Tetraphosphate - metabolism IDENTIFICATION Metabolism. Enzymes Microbiology Molecular Sequence Data Mutation ppGpp synthesis PROTEINS Pyrophosphatases - chemistry Pyrophosphatases - genetics Pyrophosphatases - metabolism SpoT protein stringent response
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Zusammenfassung:	Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293 rd position did not restore cell growth of E. coli CF8295 (Δ relA, Δ spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.66.2735