Photoacoustic determination of glucose using multilayered film

Photoacoustic determination of the glucose concentrations was conducted by using a multilayered film as a probe material for dyeing. A small amount (about 2.5 μl) of glucose solution with various concentrations (04 g/l) was dropped on one side of the film. The glucose solution permeated through the layers of the film to reach the reagent layer where the dyeing reaction occured. The dyestuff showed a characteristic absorption peak at 500 nm, and light from xenon lamp with wavelengths between 460800 was used for exciting the dyestuff. The photoacoustic signal from the other side of the film was detected. The signal intensity was found to be linear in the range from 0 to 2 g/l. This method was applied to the measurement of the glucose concentration of human blood, and was found to give somewhat higher values compared to those by mutarotase GOD method, the standard method for glucose determination. It can serve, however, as a rapid and simple diagnosis of diabetes.