A Simple Correction Method for Determination of Absolute Fluorescence Quantum Yields of Solid Samples with a Conventional Fluorescence Spectrophotometer

The absolute fluorescence quantum yield of a solid sample was measured by using a fluorescence spectrophotometer equipped with an integrating sphere. However, there were difficulties in making a spectrum correction of the integrating sphere. To solve this problem, we developed a simple method for determining its wavelength characteristics, by using the ratio of the diffuser to the integrating sphere. They were measured by combining both scan data with corrected excitation light. As a result, we were able to measure the corrected spectrum over a wide wavelength range of 200 nm to 800 nm. The experimental value of the fluorescence quantum yield of MgWO4 was 0.80 (RSD 0.6%) with the present method, which is a good agreement with a reported value (0.81).