Simultaneous Determination of Anserine and Carnosine in Chicken Meat by Hydrophilic Interaction Chromatography on an Aminopropyl Bonded Silica Gel Column

A simple separation method using hydrophilic interaction liquid chromatography (HILIC) with an ultraviolet detector (UV) on an aminopropyl (NH2) silica-gel column has been studied for the determination of anserine (ANS) and carnosine (CARN) in chicken meat. As a result of validation tests, our proposed method provides good linearity of the calibration curve as well as both repeatability and reproducibility. In addition to that, the correlation coefficient of the working curve of calibration were estimated to be from 0.9980 to 0.9984 for ANS and CARN in the concentration range from 10 pmol μL−1 to 100 pmol μL−1. The limits of detection (LOD) at 5 measurement times (N = 5), calculated on 3 σ at 10 pmol μL−1, were 0.51 pmol μL−1 for ANS, and 0.48 pmol μL−1 CARN. The limits of quantification (LOQ) at N = 5, calculated on 10 σ at 10 pmol μL−1, were 1.71 pmol μL−1 for ANS, and 1.60 pmol μL−1 for CARN. Comparing to ion-pair method and our proposals, they had a good correlation. Our proposed method could be successfully applied to the determination of ANS and CARN in chicken meat.