Determination of Glucose Oxidase by Means of the Chemiluminescence of Cypridina Luciferin Analogue

Determination of Glucose Oxidase by Means of the Chemiluminescence of Cypridina Luciferin Analogue

FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-(p-methoxyphenyl)-3, 7-dihydroimidazo[1, 2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300pmol/ml to 10nmol/ml. In a...

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Veröffentlicht in:Analytical Sciences 1994/04/10, Vol.10(2), pp.267-269
Hauptverfasser: MITANI, Motohiro, KOINUMA, Yasumi, KOSUGI, Masanori
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
chemiluminescence
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
glucose oxidase
Luciferin analogue
Oxidoreductases
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Zusammenfassung:FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-(p-methoxyphenyl)-3, 7-dihydroimidazo[1, 2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300pmol/ml to 10nmol/ml. In addition, glucose oxidase (GOD) was determined in a range from 1.18×10-5units/ml (280amol/ml) to 7.56×10-4units/ml (17.9fmol/ml) when GOD was incubated with glucose for 10min at 34°C.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.10.267