DETECTION OF GANGLIOSIDE GM1 IN PRIMARY CULTURED CELLS OF RAT BRAIN

DETECTION OF GANGLIOSIDE GM1 IN PRIMARY CULTURED CELLS OF RAT BRAIN

A new technique demonstrating the presence of ganglioside GM1 in neurons, neurite bundles and oligodendrocytes using avidin-biotin complex systems in double staining techniques is reported. The method was applied to cultured brain cells. The ganglioside GM1 may be present in the growth cones of outg...

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Veröffentlicht in:Acta histochemica et cytochemica 1985-07, Vol.18 (4), p.383
Hauptverfasser: ASOU, HIROAKI, IWASAKI-MUTOU, NORIKO, HIRANO, SHUSUKE, TURUMIZU, TAKASHI, HORIBE, YOSHIMUNE
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Sprache:eng
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Zusammenfassung:A new technique demonstrating the presence of ganglioside GM1 in neurons, neurite bundles and oligodendrocytes using avidin-biotin complex systems in double staining techniques is reported. The method was applied to cultured brain cells. The ganglioside GM1 may be present in the growth cones of outgrowing neurites in these living cell cultures.
ISSN:0044-5991
1347-5800