IMMUNOHISTOCHEMICAL LOCALIZATION OF INSULIN IN NESIDOBLASTOSIS OF RAT PANCREAS

Sprague-Dawley male rats aged 70 days were injected with Streptozotocin with nicotinamide and then administered glucocorticoid at the ages of between 150 and 230 days. Multiple hyperplasia of the nesidoblastic cells were seen often at the age of 510 days, in addition to many islet cell tumors. As well as the induced β cell tumors, the presence of insulin antigen was detected intensively in such a nesidoblastosis by the indirect immunoperoxidase technique.