Experimental Hepatic Lesions and Drug Metabolizing Enzymes in Rats

Three types of experimental hepatic lesion were produced in male rats by subacute administration of carbon tetrachloride (CCl4), 4, 4′-diaminodiphenylmethane (DDM) and by feeding low protein-high fat diet (LPHF), characterized histopathologically by centrolobular fatty changes as well as fibrosis and cirrhosis, bile duct proliferation accompanied by cell infiltration and fibrosis, and diffuse fatty changes in parenchymal cells, respectively. Microsomal oxidation enzyme systems such as P-450, aniline hydroxylase, aminopyrine N-demethylase, p-nitrophenyl acetate hydrolase (carboxyesterase) and glutathione S-alkyltransferase were inhibited in the increasing order of DDM