Synthesis of Reconstituted Hemoglobins Containing Metalloporphyrin Derivatives and SOD Activity

To establish a method for the effective mimicking superoxide dismutase (SOD) which accerelate scavenging of the superoxide anion radical (O2-·), reconstituted hemoglobins each possessing the apoprotein of hemoglobins as carriers and various metalloporphyrins as active sites were synthesized and their SOD activity was determined in each case. The hemoglobins containing iron- and manganese-protoporphyrin IX (2-4) had no significant SOD activity but did so when containing cationic iron- and manganese-porphyrins (5-7). Minimum IC50 as indicator of SOD activity was 1.8. td as indicator of hydrogen peroxide resistance was always nearly 10 times that cationic metalloporphyrins as an SOD mimic. The reconstituted hemoglobins with cationic metalloporphyrins (5-7) are shown by the present results to be potentially capable of functioning as SOD mimics.