Urinary Excretion of TTCA after Intake of brassica Vegetables

Aim: Recent studies have made it clear that brassica vegetables contain 2-thiothiazolidine-4-carboxylic acid (TTCA), which is the most widely used biological monitoring index of exposure to carbon disulfide (CS2)' This study aimed to assess the time-course of TTCA excretion in urine (TTCA-U) after eating brassica vegetables. Methods: After a 1-d break from eating brassica vegetables, ten volunteers (6 males and 4 females) ingested 100 grams of chopped raw cabbage containing 4. 3 mg/kg of TTCA, and the TTCA concentration in urine samples was determined over 24 h. TTCA concentrations in brassica vegetables purchased from a local supermarket were also measured. Results: TTCA-U reached peak concentrations 3-9 h after cabbage intake, gradually decreased, and was below the detection limit (