Genetic and Quality Identification of Alfalfa Carried by Satellite

Astronautical mutagenesis is a new and security agricultural breeding technique in recent decades. There are very large differences in physical environment between space and ground. After returning to the ground, new varieties are bred by the conventional breeding techniques. There are rich genetic types in mutagenesis. In this paper, “Longmu 801” alfalfa seeds were carried by “Shijian-8” recoverable satellite and some new types were selected and bred. The genetic variation of 11 mutated lines and their CK were identified by RAPD analysis, determination of leaf protein concentrates (LPC) and crude protein. For genetic identification, 100 primers were tested and 19 primers were selected in RAPD amplification. The amplified bands and polymorphic sites were rich. Polymorphism points and ratios of polymorphism point were statistical in detail. They were 326 and 15.04% respectively. For quality identification, it was compared with yields of alfalfa LPC and crude protein contents. The results showed that the range of yields of LPC was 6.52%-7.65%, range of nitrogen content was 43.79%-52.38%. Comparing with CK, variation ranges were -3.99%-+12.99% and -4.45%-+12.49%.