Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse

Circadian Variations in Plasma Adrenaline and Noradrenaline in the Thoroughbred Horse

Circadian variations in catecholamines (CA) in the Thoroughbred horse were investigated by determining plasma adrenaline (Ad) and noradrenaline (NA) concentrations every 2 hr by high-performance liquid chromatography with an electrochemical detector (HPLC-ED). The HPLC-ED method in the present study...

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Veröffentlicht in:Journal of Equine Science 1997, Vol.8(3), pp.81-88
Hauptverfasser: KUROSAWA, Masahiko, TAKEDA, Fujie, NAGATA, Shunichi, MIMA, Kyosuke
Format: Artikel
Sprache:eng
Schlagworte:
adrenaline
circadian variations
circulating levels
noradrenaline
Thoroughbred
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Zusammenfassung:Circadian variations in catecholamines (CA) in the Thoroughbred horse were investigated by determining plasma adrenaline (Ad) and noradrenaline (NA) concentrations every 2 hr by high-performance liquid chromatography with an electrochemical detector (HPLC-ED). The HPLC-ED method in the present study was sufficient for determining plasma CA concentrations. The elution of CA from alumina with a mixture of acetic acid and methanol (1:50, v/v) gave a good recovery. The detection limit for both Ad and NA was 10 pg/ml. A significant variation during the 24 hr was observed for plasma NA concentrations (P
ISSN:1340-3516
1347-7501
DOI:10.1294/jes.8.81