The Determination of Fluorescent Whitening Agent VBL by Fluorescence Spectroscopy

Comprehensive method for determining the fluorescent agents was studied through studying the fluorescence spectra of different concentration of VBL. The emission and excitation wavelength in the measurement was 275nm, and 434nm, respectively. When the concentrations of VBL are changing from 0 to 1.0 μg/mL, they have a linear relationship with the emitted fluorescence intensity. In this interval, the calibration curve is y= 1 6.713x+0.7879, showing strong correlation (correlation coefficient R2 is 0.9979). The average percentage of recovery is 89.9%, which meets the measuring requirements. The interaction between VBL and CTAB is preliminary studied.