Anti-Proliferative Effects on Human Colon Cancer Cells of Combined Treatment with Cationic Liposome-Entrapped Human Interferon-, [beta] or -[gamma]Gene and Anti-Cancer Drug

Effects on cultured human colon cancer cells of combination of transfection with cationic multilamellar liposome-entrapped plasmids containing cDNA of human interferon-β (hIFN-β) or interferon-γ (hIFN-γ) (RN-hIFN-γ or RN-hIFN-γ) and an anti-cancer drug (5-fluorouracil or cisplatin) were examined. At 16h after the addition of liposome-entrapped RN-hIFN-β or RN-hIFN-γ, the medium was exchanged for the fresh medium containing anti-cancer drug, of which effect of growth inhibition was strengthened synergistically, and the efficacy increased corresponding to the dose of the drug.