HDL-binding lipoproteins on human fetal hepatocytes

Two types of binding sites for high-density lipoproteins (K^sub d^=1 μg/ml and 20 μg/ml) were identified on human fetal hepatocytes and designated as P^sub 1^ and P^sub 2^. Ligand blotting has shown that P^sub 1^ site is protein with Mr 100 kD and P^sub 2^ site involves two proteins with Mr 105 and 110 kD. P^sub 2^ proteins were not detected when incubation with ligands was shortened from 24 h to 30 min. Molecular weight and activity of P^sub 1^ did not change considerably after reduction of disulfide bonds.[PUBLICATION ABSTRACT]