Analysis of hydrogen peroxide in cigarette smoke from selected Chinese cigarette brands under conventional and intense machine smoking conditions

The purpose of the present work was to investigate the concentration ranges of H 2 O 2 generated from cigarette smoke of selected Chinese brands and the effect of different smoking behaviors on the deliveries of H 2 O 2 . A simple, rapid and reliable fluorescence assay was developed for the measurem...

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Veröffentlicht in:European food research & technology 2012-12, Vol.235 (6), p.1107-1115
Hauptverfasser: Liu, Hailin, Sun, Shihao, Zong, Yongli, Li, Peng, Xie, Jianping
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creator Liu, Hailin
Sun, Shihao
Zong, Yongli
Li, Peng
Xie, Jianping
description The purpose of the present work was to investigate the concentration ranges of H 2 O 2 generated from cigarette smoke of selected Chinese brands and the effect of different smoking behaviors on the deliveries of H 2 O 2 . A simple, rapid and reliable fluorescence assay was developed for the measurement of H 2 O 2 based on the catalytic activity of hemoglobin using 10-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red) as the substrate. The proposed method was applied to determine the H 2 O 2 deliveries from mainstream cigarette smoke of 30 Chinese (5 blended and 25 Virginia) and two reference cigarettes (3R4F and 1R5F) under two machine smoking regimes. Results showed that, under ISO smoking conditions, Chinese blended cigarettes had lower H 2 O 2 yields than Chinese flue-cured cigarettes (mean value 2.41 vs. 4.69 μg cig −1 , p  
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A simple, rapid and reliable fluorescence assay was developed for the measurement of H 2 O 2 based on the catalytic activity of hemoglobin using 10-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red) as the substrate. The proposed method was applied to determine the H 2 O 2 deliveries from mainstream cigarette smoke of 30 Chinese (5 blended and 25 Virginia) and two reference cigarettes (3R4F and 1R5F) under two machine smoking regimes. Results showed that, under ISO smoking conditions, Chinese blended cigarettes had lower H 2 O 2 yields than Chinese flue-cured cigarettes (mean value 2.41 vs. 4.69 μg cig −1 , p  &lt; 0.05). As expected, the deliveries of H 2 O 2 for all cigarettes analyzed were higher under Canadian intense smoking conditions than those under ISO conditions (ISO mean 4.31 μg cig −1 vs. Canadian mean 5.96 μg cig −1 , p  &lt; 0.05). In addition, the ratios of H 2 O 2 to nicotine yields were utilized to show the relative differences in yields among brands tested in this work and more than a twofold difference was found for most of selected Chinese blended and flue-cured cigarettes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s00217-012-1840-6</doi><tpages>9</tpages></addata></record>
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subjects Agriculture
Analytical Chemistry
Behavior
Biological and medical sciences
Biotechnology
Brands
Chemistry
Chemistry and Materials Science
Cigarettes
Enzymes
Food industries
Food Science
Forestry
Fundamental and applied biological sciences. Psychology
Hemoglobin
Hydrogen peroxide
Laboratories
Nicotine
Original Paper
Reagents
Smoking
Statistical analysis
Tobacco
Ventilation
title Analysis of hydrogen peroxide in cigarette smoke from selected Chinese cigarette brands under conventional and intense machine smoking conditions
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