Analysis of hydrogen peroxide in cigarette smoke from selected Chinese cigarette brands under conventional and intense machine smoking conditions

The purpose of the present work was to investigate the concentration ranges of H 2 O 2 generated from cigarette smoke of selected Chinese brands and the effect of different smoking behaviors on the deliveries of H 2 O 2 . A simple, rapid and reliable fluorescence assay was developed for the measurement of H 2 O 2 based on the catalytic activity of hemoglobin using 10-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red) as the substrate. The proposed method was applied to determine the H 2 O 2 deliveries from mainstream cigarette smoke of 30 Chinese (5 blended and 25 Virginia) and two reference cigarettes (3R4F and 1R5F) under two machine smoking regimes. Results showed that, under ISO smoking conditions, Chinese blended cigarettes had lower H 2 O 2 yields than Chinese flue-cured cigarettes (mean value 2.41 vs. 4.69 μg cig −1 , p