Functionalization of ultradispersed diamond for DNA detection
Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizi...
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| Veröffentlicht in: | Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2008-12, Vol.10 (Suppl 1), p.69-75 |
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| container_end_page | 75 |
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| container_issue | Suppl 1 |
| container_start_page | 69 |
| container_title | Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology |
| container_volume | 10 |
| creator | Yang, Jung-Hoon Nakano, Yoshikazu Murakami, Yasunori Song, Kwang-Soup Kawarada, Hiroshi |
| description | Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy. |
| doi_str_mv | 10.1007/s11051-008-9367-2 |
| format | Article |
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For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.</description><identifier>ISSN: 1388-0764</identifier><identifier>EISSN: 1572-896X</identifier><identifier>DOI: 10.1007/s11051-008-9367-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biotechnology ; Characterization and Evaluation of Materials ; Chemistry and Materials Science ; Deoxyribonucleic acid ; DNA ; Fluorescence microscopy ; Inorganic Chemistry ; Lasers ; Materials Science ; Nanoparticles ; Nanotechnology ; Optical Devices ; Optics ; Photonics ; Physical Chemistry ; Proteins ; Research Paper</subject><ispartof>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology, 2008-12, Vol.10 (Suppl 1), p.69-75</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-d0f3e4c4c9207a9236896f4b9e31e6a7ff955ec1808f3825abcf1b44147ac10b3</citedby><cites>FETCH-LOGICAL-c382t-d0f3e4c4c9207a9236896f4b9e31e6a7ff955ec1808f3825abcf1b44147ac10b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11051-008-9367-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11051-008-9367-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Yang, Jung-Hoon</creatorcontrib><creatorcontrib>Nakano, Yoshikazu</creatorcontrib><creatorcontrib>Murakami, Yasunori</creatorcontrib><creatorcontrib>Song, Kwang-Soup</creatorcontrib><creatorcontrib>Kawarada, Hiroshi</creatorcontrib><title>Functionalization of ultradispersed diamond for DNA detection</title><title>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology</title><addtitle>J Nanopart Res</addtitle><description>Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. 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In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.</description><subject>Biotechnology</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry and Materials Science</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fluorescence microscopy</subject><subject>Inorganic Chemistry</subject><subject>Lasers</subject><subject>Materials Science</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Photonics</subject><subject>Physical Chemistry</subject><subject>Proteins</subject><subject>Research 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detection</atitle><jtitle>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology</jtitle><stitle>J Nanopart Res</stitle><date>2008-12</date><risdate>2008</risdate><volume>10</volume><issue>Suppl 1</issue><spage>69</spage><epage>75</epage><pages>69-75</pages><issn>1388-0764</issn><eissn>1572-896X</eissn><abstract>Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11051-008-9367-2</doi><tpages>7</tpages></addata></record> |
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| subjects | Biotechnology Characterization and Evaluation of Materials Chemistry and Materials Science Deoxyribonucleic acid DNA Fluorescence microscopy Inorganic Chemistry Lasers Materials Science Nanoparticles Nanotechnology Optical Devices Optics Photonics Physical Chemistry Proteins Research Paper |
| title | Functionalization of ultradispersed diamond for DNA detection |
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