Functionalization of ultradispersed diamond for DNA detection

Functionalization of ultradispersed diamond for DNA detection

Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizi...

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Veröffentlicht in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2008-12, Vol.10 (Suppl 1), p.69-75
Hauptverfasser: Yang, Jung-Hoon, Nakano, Yoshikazu, Murakami, Yasunori, Song, Kwang-Soup, Kawarada, Hiroshi
Format: Artikel
Sprache:eng
Schlagworte:
Biotechnology
Characterization and Evaluation of Materials
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
Fluorescence microscopy
Inorganic Chemistry
Lasers
Materials Science
Nanoparticles
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Proteins
Research Paper
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Zusammenfassung:Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-008-9367-2