Kupffer cell–derived Fas Ligand plays a role in liver injury and hepatocyte death

Kupffer cell–derived Fas Ligand plays a role in liver injury and hepatocyte death

Liver injury is an important prognostic indicator during acute pancreatitis. The aim of this study was to determine the role of Fas ligand (FasL) in hepatocyte injury. Liver parenchymal enzymes were measured in cocultures of hepatocytes and Kupffer cells treated with elastase. FasL and FasL mRNA wer...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of gastrointestinal surgery 2004-02, Vol.8 (2), p.166-174
Hauptverfasser: Yang, Jun, Gallagher, Scott F, Haines, Krista, Epling-Burnette, P.K, Bai, Fenqi, Gower, William R, Mastorides, Stephen, Norman, James G, Murr, Michel M
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Apoptosis - physiology
Caspase 3
Caspases - metabolism
Cells
Cells, Cultured
Fas ligand
Fas Ligand Protein
hepatocyte apoptosis
Hepatocytes - drug effects
Hepatocytes - physiology
Kinases
Kupffer cells
Kupffer Cells - drug effects
Kupffer Cells - metabolism
Liver
Liver Diseases - metabolism
liver injury
Male
Membrane Glycoproteins - pharmacology
Mitogen-Activated Protein Kinases - metabolism
Models, Animal
Pancreatitis
Rats
Rats, Sprague-Dawley
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Liver injury is an important prognostic indicator during acute pancreatitis. The aim of this study was to determine the role of Fas ligand (FasL) in hepatocyte injury. Liver parenchymal enzymes were measured in cocultures of hepatocytes and Kupffer cells treated with elastase. FasL and FasL mRNA were measured in elastase-treated Kupffer cells. Hepatocytes were treated with FasL and their viability was assessed by monotetrazolium (MTT), apoptosis by flow cytometry, as well as caspase-3 and p38–mitogen-activated protein kinase (MAPK) by immunoblotting. Elastase increased aspartate aminotransferase and lactate dehydrogenase in cocultures of hepatocyte and Kupffer cells ( P
ISSN:1091-255X
1873-4626
DOI:10.1016/j.gassur.2003.10.016