Validation of an Elisa according to EC decision 2002/657/EC for detection of zearalenone in feedstuff

This study presents data from a single laboratory for internal validation of an enzyme-linked immunoassorbent assay (ELISA) for determination of levels of the mycotoxin zearalenone in complementary and complete feedstuff for dairy cattle, calves, sheep and goats. the study was carried out at the request of European commission (EC) recommendation 576/2006/EC, in agreement with EC Decision 2002/657/EC on the implementation of EC Directive 96/23/EC and concerning the performance of analytical methods and interpretation of results. this screening detection method has useful advantages: it can analyse large numbers of samples in a relatively short time, with a small quantity of sample required; it is sufficiently robust, reliable and rugged to be statistically sound; and it generates few suspected false negatives (< 5%). --Keywords: animal feed, EC Decision 2002/657/EC, ELISA, mycotoxin, validation, zearalenone--