In vitro effects of bacterial lipopolysaccharide (LPS) on motion parameters of ram epididymal sperm

This study investigated in vitro effects of bacterial lipopolysaccharide (LPS) on ram sperm motion parameters at concentrations less than its spermicidal effects. After incubation of epididymal sperm samples for 45, 90, 135, 180, and 225 min in the presence of LPS (50, 100, 200, and 400 µg/ml), motion parameters were evaluated by computer-aided sperm analysis. There was no significant increase of the fast progressive motility (class A) of sperm in LPS-treated groups at different times while there was a decrease in the slow progressive motility (class B) and the non-progressive motility (class C) of sperm in LPS-treated groups at different times that were only significant (P