Immunocytochemical identification of CD5 positive peripheral blood lymphocytes in four marsupial species
Immunocytochemical analysis of peripheral blood mononuclear cells was undertaken using a streptavidin biotin-horseradish peroxidase method to detect CD5 positive lymphocytes from the blood of several marsupial species. A monoclonal antibody raised to a conserved peptide sequence of the human CD5 ant...
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Veröffentlicht in: | Comparative clinical pathology 2003-05, Vol.12 (1), p.26-32 |
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Sprache: | eng |
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Zusammenfassung: | Immunocytochemical analysis of peripheral blood mononuclear cells was undertaken using a streptavidin biotin-horseradish peroxidase method to detect CD5 positive lymphocytes from the blood of several marsupial species. A monoclonal antibody raised to a conserved peptide sequence of the human CD5 antigen positively labelled lymphocytes in freshly isolated peripheral blood mononuclear cells of the tammar wallaby (Macropus eugenii), the long-footed potoroo (Potorous longipes), the long-nosed potoroo (Potorous tridactylus) and the rufous hare-wallaby (Lagorchestes hirsutus). A polyclonal anti-CD3 antibody also positively labelled circulating lymphocytes from the tammar wallaby. Whereas previous studies using flow cytometry reported labelling of T cells in koala lymphocyte preparations using a polyclonal anti-CD3 antibody, there have been no other reports of marsupial blood immunophenotyping. The current study extends the known applications of monoclonal anti-CD5 and polyclonal anti-CD3 antibodies to blood lymphocytes of small wallaby species using an immunocytochemical slide technique that is simple, can be processed within a day and requires no dedicated large equipment.[PUBLICATION ABSTRACT] |
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ISSN: | 1618-5641 1618-565X |
DOI: | 10.1007/s00580-002-0465-8 |