P-527: The impact of glucose-loading on endothelial function and inflammatory process in patients diabetes mellitus type 2: Effects of diet and hypoglycemic agents

Background: The role of glucose alterations in the pathogenesis of endothelial dysfunction and inflammatory process in diabetic patients remains unclear. We examined the effect of diet or metformin on endothelial function and inflammatory process during glucose loading, in patients with newly diagnosed diabetes mellitus. Methods: The study consisted of 20 patients with newly diagnosed diabetes mellitus type 2, mean age 60.2±2.7, who underwent glucose loading (75gr glucose) at baseline and 3 months after dietary instructions (n=12, group A) or after daily metformine administration for (n=8, group B). Blood samples were taken and gauge-strain plethysmography was performed at baseline and 3 hours after loading, while the protocol was performed after 3 months treatment period. Endothelium dependent dilation (EDD) and endothelium-independent dilation (EID) were expressed as the % change of flow from baseline to the maximum flow during reactive hyperemia or after sublingual nitroglycerin administration respectively. Results: In group B, EDD was significantly decreased at 1h (from 67±27% to 54±5%, p