P-490: PPAR-g2 Pro12Ala and the b3- adrenergic receptor Trp64Arg polymorphisms in Brazilian subjects: comparison between obese hypertensives and non obese individuals

This study has been conducted to test the hypothesis that obesity and hypertension are related to polymorphisms on PPAR-γ2 and β3-adrenergic receptor genes. DNA samples from a hundred obese hypertensive patients [27 males, 73 females, age 20–65 yrs, BMI > 30.0 kg/m2], and 100 lean healthy individuals (50 males and 50 females, age 20–65 yrs, BMI < 25 kg/m2). Genomic DNA was extracted from peripheral blood leukocytes using the salting-out method and amplified by polymerase chain reaction (PCR). The PCR products were digested with the enzyme BstUI and BstNI for detection of PPAR-γ2 Pro12Ala and β3-adrenergic receptor Trp64Arg polymorphisms respectively, during 3h at 60°C. The digested samples were separated by electrophoresis through an 8% polyacrilamide gel and visualized by staining with silver nitrate. We used the Fischer test to analyze our data results. Fourteen obese hypertensive individuals and eight lean individuals presented PPAR-γ2 Pro12Ala polymorphism and eleven obese hypertensives and ten lean individuals presented β3- adrenergic receptor Trp64Arg polymorphism. The frequency of both polymorphisms didn't differ between the groups: OR = 1.88 (C.I. = 0.75–4.68; p = 0.25) for the PPAR-γ2 Pro12Ala polymorphism and OR= 1.11 (C.I. = 0.45–2.75; p = 1.0) for the β3-adrenergic receptor Trp64Arg polymorphism. The frequencies of the PPAR-γ2 Pro12Ala and the β3-adrenergic receptor Trp64Arg polymorphisms among obese hypertensive and lean individuals did not differ in our preliminary sample of a Brazilian population. This project was supported by Grant-in-Aid from Glaxo-Wellcome and Internal Medical Assistance Limited -AMIL. Am J Hypertens (2004) 17, 212A–212A; doi: 10.1016/j.amjhyper.2004.03.564