On-Site Assay by Portable UV Detector: MC-CE Device for Authenification of Chinese Medicine

Wan by CE and Portable MC-CE Device ... 215 12.2 Procedures and Operations ... 21512.2.1 Reagent and Buffer Preparation ... 215 12.2.2 Preparation of Liu Wei Di Huang Wan Herbal Medicine ... 215 12.2.3 Extraction Procedures... 216 12.2.4 Operation Procedures for CE and MC-CE Device ... 21812.3 Optimization of CE Separation Procedures for Fingerprinting of Liu Wei Di Huang Wan ... 218 12.3.1 Effect of Methanol Content ... 218 12.3.2 Effect of SDS Concentration ... 219 12.3.3 Effect of Borate Concentration ... 219 12.3.4 Effect of pH ... 220 12.3.5 Effect of CE Operation Conditions ... 22012.4 Results for Fingerprinting of Liu Wei Di Huang Wan by CE Methods ...221 12.4.1 Qualitative Fingerprinting by Electropherogram ... 221 12.4.2 Quantitative Fingerprinting of Liu Wei Di Huang Wan ... 22612.5 Authenication of Liu Wei Di Huang Wan by MC-CE Device with Portable UV Detector ... 232 12.5.1 Instrumentation and Facilities ... 232The increasing use of herbs for medicinal purpose in recent years leads to the requirement for assessing their quality to assist international trading of herbal products. Herbs are complex products with variable contents, depending on sources and season for their harvesting. The analytical issue for assessing the quality of herbal medicine is addressed in this chapter and current practices using ngerprinting are reviewed in Section 12.1.1.